• N,N-双(2-羟乙基)甘氨酸 ,99%
  • C04006 150-25-4

    N,N-Bis(2-hydroxyethyl)glycine

产品订购
产品货号重量货期 优惠价 数量
C04006-100g 100g 准现货 询价
C04006-25g 25g 准现货 询价
C04006-500g 500g 准现货 询价
  • 产品信息

属性

溶解性 Soluble in water (1M) at 20 °C, ethanol (slightly), ether (slightly), and chloroform (slightly).
存贮条件 室温保存
熔点 190-192°C
密度 1.0500
荧光
IC50
pK Values

描述

别名 N,N-二(2-羟乙基)氨基乙酸;N,N-二羟乙基甘氨酸;二乙醇甘氨酸;二羟乙甘胺酸;Bicine; Diethylolglycine; N,N-Di-(Hydroxyethyl) glycine; N,N-Bis(2-hydroxyethyl)glycine
产品介绍 Bicine is a zwitterionic amino acid buffer, active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Recommended buffer for low temperature biochemical work. Bicine is used for the preparation of stable substrate solution for serum guanase determination. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.
用途 生物缓冲剂低温生化工作中推荐buffer。用在制备稳定的底物溶液,用于血清鸟嘌呤酶测定。也是用途非常广泛的一种Buffer,用于酶反应缓冲液,电泳缓冲液,工作浓度从3-100mM。

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