• 邻硝基苯-β-D-吡喃半乳糖苷,超纯,99%
  • D120081 369-07-3

    ONPG

产品订购
产品货号重量货期 优惠价 数量
D120081-1g 1g 准现货 询价
D120081-25g 25g 准现货 询价
D120081-5g 5g 准现货 询价
D120081-100g 100g 准现货 询价
  • 产品信息

属性

存贮条件 -20℃充氩保存
熔点 195°C
密度 1.5990
旋光率 -68.8 ° (C=1, H2O)
荧光
IC50
pK Values

描述

别名 ONPG;邻硝基苯-β-D-吡喃半乳糖苷;o-Nitrophenyl β-D-galactopyranosideONPG
产品介绍 溶于水、甲醇,水中溶解度可达15mg/ml。有吸湿性。
用途 用于检测β-半乳糖苷酶(β-galactosidase)活性,是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物,在410-420nm波长可以检测吸光度。
生化机理 β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

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