别名
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ONPG;邻硝基苯-β-D-吡喃半乳糖苷;o-Nitrophenyl β-D-galactopyranosideONPG
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产品介绍
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溶于水、甲醇,水中溶解度可达15mg/ml。有吸湿性。
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用途
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用于检测β-半乳糖苷酶(β-galactosidase)活性,是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物,在410-420nm波长可以检测吸光度。
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生化机理
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β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
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