• 核糖核酸酶T1,BR,1000u/ul
  • G63249 9026-12-4

    Rnase T1

产品订购
产品货号重量货期 优惠价 数量
G63249-100KU 100KU 准现货 询价
G63249-500KU 500KU 准现货 询价
  • 产品信息

属性

存贮条件 储存温度 2-8°C
密度
荧光
IC50
pK Values

描述

别名 RNase T1;Guanyloribonuclease;Ribonucleate 3‘-guanylo-oligonucleotidohydrolase
产品介绍 Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3‘-phosphate residues (or inosine 3‘-phosphate) and the 5‘-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2‘, 3‘-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3‘-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.
用途 Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .
生化机理 Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3‘-GMP and oligonucleotides with a 3‘-GMP terminal group.

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