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  2. 酶类
  • 磷酸二酯酶I,BR,20u/mg

  • G63387 9025-82-5

    Phosphodiesterase I from Crotalus adamanteus venom

产品订购
产品货号重量货期 优惠价 数量
G63387-100U 100U 准现货 询价
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  • 产品信息

属性

存贮条件 储存温度 -20°C
密度 否
荧光 否
IC50 否
pK Values 否

描述

别名 磷酸二脂酶I;5‘-Exonuclease;Oligonucleate 5‘-nucleotidohydrolase
产品介绍 Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5‘-mononucleotides from 3‘-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.
用途 Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP.
生化机理 Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

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