蛋白酶S，BR，500u/mg,elisa试剂盒,elisa检测试剂盒,上海晶抗生物工程有限公司

产品货号	重量	货期	优惠价	数量
G63391-1mg	1mg	准现货	询价
G63391-5mg	5mg	准现货	询价

产品货号

重量

货期

优惠价

数量

G63391-1mg

1mg

准现货

询价

G63391-5mg

5mg

准现货

询价

存贮条件	储存温度 2-8°C
密度	否
荧光	否
IC50	否
pK Values	否

存贮条件

储存温度 2-8°C

密度

否

荧光

否

IC50

否

pK Values

否

别名	蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8;Endoproteinase Glu-C from Staphylococcus aureus V8;Protease,Staph aureus from Staphylococcus aureus V8
产品介绍	Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH‘s of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).
用途	Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping .
生化机理	Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.
备注	Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder.

别名

蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8;Endoproteinase Glu-C from Staphylococcus aureus V8;Protease,Staph aureus from Staphylococcus aureus V8

产品介绍

Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH‘s of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).

用途

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping .

生化机理

Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.

备注

Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder.

标识符号	GHS07, GHS08
信号词	Danger
危害声明	H315,H317,H319,H334,H335
警示性声明	P261,P280,P305+P351+P338,P342+P311
个人防护设备	dust mask type N95 (US), Eyeshields, Faceshields, Gloves
危害码（欧洲）	Xn
风险声明 (欧洲)	R36/37/38;R42/43
安全声明（欧洲）	S22;S26;S36/37;S45
RIDADR	否
WGK德国	3
闪点（华氏）	N/A
是否危化品	否

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