属性

密度 1.1700
熔点 98°C-100°C
存贮条件 储存温度 2-8°C
描述

产品介绍 AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .
别名 AEC, 50X ;3-氨基-N-乙基咔唑;AEC, 50X
生化机理 3-氨基-9-乙基咔唑是一种过氧化酶底物，适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性红色的末端产物。肉眼或可见光下就可观察。
应用 过氧化物酶底物.
Usage
? Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.
? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;).
? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.
? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.
? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.
? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; )
? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations