属性

敏感性 易吸潮,对热及光线敏感
熔点 231°C
溶解性 Soluble in water (4 mg/ml), ethanol (8 mg/ml), ethylene glycol (30 mg/ml), DMSO (slightly), and methanol: water (1:1, 50 mg/ml, very faintly turbid, very deep yellow,hot).
存贮条件 储存温度2-8℃
描述

产品介绍 Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity.
别名 碘硝基氯化四氮唑蓝;2-(对碘苯基)-3-(对硝基苯基)-5-苯基四唑氯水合物;2-(4-碘苯基)-3-(4-硝基苯基)-5-苯基氯化四唑氯水合物;碘硝基氯化四氮唑;INT;2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; p-Iodonitrotetrazolium Violet; INT Iodonitrotetrazolium chloride
生化机理 spectrophotometric assay for succinate dehydrogenase activity: Materials: spectrophotometer cuvette with 1-cm light patch. Reaction Mixture: 100 mmol/liter triethanolamine (HCl), pH8,3 o.5 mmol/liter EDTA, 2 mmol/liter Iodonitrotetrazolium chloride, 12 g/liter Cremophor EL, 20 mmol/liter succinate, pH7.4 Reaction: mix 990 ul of reaction mixture with 10ul mitochondrial suspension color develops at 30oC record for 6 min. @500 nm
应用 色度法检测各种脱氢酶的电子受体（Electron acceptor）