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别名
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己糖激酶;ATP:D-Hexose-6-phosphotransferase;Hexokinase from Saccharomyces cerevisiae;Hexokinase
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产品介绍
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Hexokinase catalyzes the reaction:D-hexose + ATP → D-hexose 6-phosphate + ADPHexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973).Hexokinase is used to determine glucose, fructose, mannose and ATP.
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用途
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Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.
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生化机理
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Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C):D-fructose KM: 0.33 mM;D-glucose KM: 0.12 mM;D-mannose KM: 0.05 mM.Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.Molecular Weight: ~ 54 kDa (monomer);~110 kDa (dimer);Optimal pH: 7.5 to 9.0;Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm.Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose?, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate).
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021-54720761
021-54720761
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