• 脱氧核糖核酸酶Ⅱ(猪脾),BR,2000-6000U/mg,TypeVI
  • G63253 9025-64-3

    DNase II

产品订购
产品货号重量货期 优惠价 数量
G63253-30KU 30KU 准现货 询价
  • 产品信息

属性

存贮条件 储存温度2-8℃
密度
荧光
IC50
pK Values

描述

别名 ;DNase II;Deoxyribonucleate 3‘-oligonucleotido-hydrolase
产品介绍 Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3‘-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
用途 DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
生化机理 The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3‘-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.

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