英文名称 : Glutathione Reductase Activation Coefficient Assay Kit
产品包装 : 盒装
产品规格 : 50T/24S
储存条件 : -20℃
检测方法 :可见分光光度法
有 效 期 : 6个月
产品组成 :
试剂名称
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规格
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保存条件
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试剂一
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液体40 mL×1瓶
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2-8℃保存
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试剂二
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粉剂×1支
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2-8℃保存
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试剂三 A
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粉剂×1支
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-20℃保存
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试剂三 B
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液体1mL×1支
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2-8℃保存
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试剂四
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液体1.2mL×1支
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2-8℃保存
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试剂五
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粉剂×1支
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-20℃保存
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试剂六
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液体20 mL×1瓶
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2-8℃保存
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试剂七
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液体50 mL×1瓶
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2-8℃保存
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溶液的配制:
1.试剂二:临用前加入1.2mL蒸馏水,充分溶解,2-8℃可保存4周;
2. 试剂三:临用前取试剂三A加入0.537mL试剂三B,充分溶解,-20℃可分装保存4周,避免反复冻融;
3. 工作液:临用前根据样本量按照试剂一:试剂二:试剂三:试剂四=80μL:20μL:8μL:20μL(128μL,1T)的比例配制工作液,现用现配;
4. 试剂五:临用前加入1.2mL蒸馏水,充分溶解,-20℃可分装保存4周,避免反复冻融;
5. 试剂五工作液:临用前根据样本量按照试剂五:蒸馏水=5μL:95μL(0.1mL,5T)的比例配制,现用现配。
产品说明:
谷胱甘肽还原酶(GlutathioneReductase,GR)是广泛存在于真核与原核生物中的一种黄素蛋白氧化还原酶,该酶以核黄素衍生物核黄素腺嘌呤二核苷酸(FAD)为辅基,催化NADPH还原氧化型谷胱甘肽(GSSG)生成还原型谷胱甘肽(GSH),维持巯基和膜蛋白处于还原状态。当生物体内缺乏核黄素时,FAD含量相应减少,GR活性也随之降低,谷胱甘肽还原酶活性系数(GRActivationCoefficient,GRAC)迅速升高,出现唇炎、口角炎、结膜炎等临床症状。因此,GRAC用于核黄素营养状况评价,对于早期发现缺乏病患者采取防治措施具有重要意义。GR催化NADPH还原GSSG,生成GSH,GSH与5,5’-二硫代-双-(2-硝基苯甲酸)(5,5’-dithiobis-2-nitrobenoicacid,DTNB)反应产生2-硝基-5-巯基苯甲酸,2-硝基-5-巯基苯甲酸在波长412nm处有特征吸收峰,通过412nm处吸光度的变化可计算GR的活性。根据加入FAD前后GR活性的变化可计算得到GRAC。
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)
注意 :实验之前建议选择2-3个预期差异大的样本做预实验。如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
需自备的仪器和用品:
可见分光光度计、低温离心机、分析天平、水浴锅/恒温培养箱、1mL玻璃比色皿、可调式移液枪、研钵/匀浆器/细胞超声破碎仪、冰和蒸馏水。
操作步骤 :
一、样本处理(可适当调整待测样本量)
1.组织样本:按质量(g):试剂一体积(mL)=1:5~10比例加入试剂一(建议称取0.1g样本,加入1.0mL试剂一),冰浴匀浆后,于4℃,12000rpm离心10min,弃沉淀,取上清液置于冰上待测。
2.细胞样本:按细胞数量(104):试剂一体积(mL)=500~1000:1的比例加入试剂一(建议500万细胞加入1.0mL试剂一),冰浴超声破碎细胞(功率200W,超声3s,间隔7s,总时间3min),然后于4℃,12000rpm离心10min,弃沉淀,取上清液置于冰上待测。
3.全血/红细胞:建议取10μL全血/红细胞,加入990μL试剂一,充分溶血混匀,于4℃,4000rpm离心10min,弃沉淀,取上清液置于冰上待测。
4.血清/血浆等液体样本:直接测定。若有浑浊请离心后取上清置于冰上待测。